Stichodactyla Toxin peptide (Atto 590)
Reactivity: Stichodactyla helianthus
Host: Synthetic
BP, FACS, IF, LCI
Catalog No. ABIN7582282
Quick Overview for Stichodactyla Toxin peptide (Atto 590) (ABIN7582282)
Target
Stichodactyla Toxin
Origin
Stichodactyla helianthus
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 590
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Purpose
- Fluorescent ShK Conjugate for Sensitive Detection of KV Channels
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Sequence
- RSCIDTIPKS RCTAFQCKHS MKYRLSFCRK TCGTC-OH
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Specificity
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Toxin target: KV1.1, KV1.3, KV1.4, KV1.6 channels,
Disulfide bonds between: Cys3-Cys35, Cys12-Cys28, Cys17-Cys32 ATTO Fluor-590 -
Characteristics
- Fluorescent ShK Conjugate for Sensitive Detection of KV Channels
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Stichodactyla Toxin peptide (Biotin)
Reactivity: Stichodactyla helianthus Host: Synthetic BP, FACS, IF
Stichodactyla Toxin peptideReactivity: Stichodactyla helianthus Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 1 - 100 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- Stichodactyla Toxin
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Background
- K+ channel toxin ShK, Toxin κ-stichotoxin-She3a, Kappa-stichotoxin-She3a, κ-SHTX-She3a,ShK (Stichodactyla Toxin) is a peptide toxin originally isolated from the nematocyst of the sea anemone Stichodactyla helianthus1.ShK blocks KV1.3, KV1.1, KV1.4, and KV1.6 at subnanomolar concentrations and KV3.2 channels at 1000-fold higher concentration than that required to inhibit KV1.3 channels. It has been shown to block KV current in DRG neurons, to displace radioactive Dendrotoxin from brain synaptosomes1 and inhibit I125-Charybdotoxin binding to Jurkat T lymphocytes with an IC50 of 32 pM2,4.Evidence also suggests that native KV currents in the central nervous system, which are predominantly carried by KV1.2 channels, are highly sensitive to this toxin5.A fluorescently labeled synthetic ShK is used to recognize and to sort KV1.3 containing lymphocytes4,6.
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Molecular Weight
- ~4627 Da
Target
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